D4889

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Genomic, unsheared

• Synonyme(s) : DNA
• CAS Number: 93384-16-8
• Numéro CE : 297-226-7
• Numéro MDL: MFCD00130922
• Code UNSPSC : 41106310
• eCl@ss : 32160414
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Forme: lyophilized powder
• Poids mol.: >45 kb
• Température de stockage: 2-8°C

Description

Description générale

Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.

Spécificité

50% GC; T_m = 90.5 °C

Application

The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:

• PCR reactions of genomic DNA of Gardnerella vaginalis
• nick translation and radiolabeling of dNTP
• generating standard curve for 16S rDNA quantification
• transfecting neutrophils
• testing the extraction methods

Définition de l'unité

One unit will yield an A₂₆₀ of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).

Notes préparatoires

Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A₂₆₀ units per mg DNA. % GC is the percentage of G/C base pairs. T_m is the temperature at the midpoint of the thermal denaturation profile.

Remarque sur l'analyse

Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.

Autres remarques

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)