SRE0069

Phosphoglucomutase from rabbit muscle

• Synonyme(s) : α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• CAS Number: 9001-81-4
• Numéro CE : 5.4.2.2 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131886
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: ≥100 units/mg protein
• Activité étrangère: Lactic dehydroenase ≤0.5%; Phosphoglucose Isomerase ≤0.01%; pyruvate kinase ≤0.05%
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Research area: CellSignalling. The phosphoglucomutase (PGM) molecule, a single polypeptide chain, is made up of four α/β domains. The domains form a compact heart-shaped structure with a large fissure between the two lobes, and the molecule comprises about 40 secondary structural components. The active enzymatic site of the molecule is located at the bottom of the crevice on the surface of domain I. Rabbit muscle phosphoglucomutase (RB-PGM) is a monomer with a unique four-domain architecture.

Application

Phosphoglucomutase from rabbit muscle may be used in dephosphorylation to investigate the hexose bisphosphate activation of phosphoglucomutase.

Actions biochimiques/physiologiques

Phosphoglucomutase (PGM) mainly catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate via a glucose 1,6-diphosphate intermediate. Hence it plays a key role in glycolysis and gluconeogenesis.

Définition de l'unité

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Forme physique

Lyophilized powder containing Tris HCl, EDTA, magnesium acetate and carbohydrate

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable