P2621

Phosphomannose Isomerase from Escherichia coli

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein

• 동의어(들): D-Mannose-6-phosphate ketol-isomerase, Mannose Phosphate Isomerase, PMI
• EC 번호: 5.3.1.8 (BRENDA, IUBMB)
• MDL number: MFCD00131899
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥50 units/mg protein
• 저장 온도: 2-8°C

설명

애플리케이션

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

생화학적/생리학적 작용

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

단위 정의

One unit will convert 1.0 μmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH 7.6 at 25 °C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

물리적 형태

Supplied as a suspension in 3.2 M ammonium sulfate

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)