EPI007

Histone Deacetylase 8 (HDAC8) Inhibitor Screening Kit

100 assays in 96 well plates

• Numéro CE : 3.5.1.98 (BRENDA, IUBMB)
• Code UNSPSC : 41116158
• Nomenclature NACRES : NA.41

Propriétés

• Utilisation: 100 assays in 96 well plates
• Numéro d'accès NCBI: NM_001166418 (Human); NM_027382 (Mouse)
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... HDAC8(55869) ; mouse ... HDAC8(70315)

Description

Description générale

Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, HDACs play a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.

With Sigma′s HDAC8 Inhibitor Screening Kit, HDAC8 Enzyme acts with the supplied Developer to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity releases the quenched fluorescent group, AFC, which can be detected at Em/Ex=380/500 nm. In the presence of a HDAC8 inhibitor, AFC is not released and its fluorescence remains quenched. The kit provides a rapid, simple, sensitive, and reliable test, suitable for either individual tests or high throughput screening of HDAC8 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare with the efficacy of test inhibitors.

Caractéristiques et avantages

• Simple, sensitive, and reliable assay
• Simple procedure; takes ~60 min
• Utilizes fluorometric methods
• Sample type: candidate HDAC8 inhibitors
• Suitable for screening HDAC8 inhibitors
• Suitable for individual tests or high throughput assays
• Convenient 96-well microplate format

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 188.6 °F - closed cup
• Point d'éclair (°C): 87 °C - closed cup