C8058

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

• 동의어(들): Chondroitin sulfate B lyase
• CAS Number: 52227-83-5
• MDL number: MFCD00166444
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Flavobacterium heparinum)
• Quality Level: 200
• 결합: (Glucosaminoglycan)
• 양식: lyophilized powder (with BSA as stabilizer)
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

애플리케이션

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.

The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.

생화학적/생리학적 작용

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co²⁺, Fe³⁺ and Ba²⁺ also inhibit its activity.

단위 정의

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319
• 예방조치 성명서: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)